We then grouped significant factors to analyze prognostic factors using log-rank test and revealed that age, tumor differentiation, AJCC stage, lymph node metastasis, distant metastasis, CD133, BMI-1, and co-expression of CD133 and BMI-1 were all significant prognostic indicators for overall survival of pancreatic cancer patients (P<0.05, Table 3). The gene discussed is PROM1; the disease is familial pancreatic carcinoma.