Infection of this PR1 VIGS-targeted construct (attBSMV:PR1) produced a moderate phenotypic bleaching in ‘Fielder’ spikes (Additional file 1: Figure S1) accompanied by the relative reduction of PR1 gene expression by approximately 70 % compared to attBSMV:00 construct at 7 days post inoculation (Fig. 5a). The gene discussed is TMEM37; the disease is infection.