Fluorescence information on Ki67, CK and DAPI was simultaneously and randomly obtained based on 3 fields for each BC tissue sections under Olympus BX51 fluorescent microscope (Olympus Optical Co., Ltd. Tokyo, Japan) equipped with CRi Nuance multispectral imaging system (Cambridge Research and Instrumentation, Inc., Woburn, MA, USA) at 400 × magnifications. This evidence concerns the gene MKI67 and breast cancer.