At first, the mixture of primary antibodies of anti-Ki67 rabbit monoclonal antibody (Clone: SP6; dilution: 1:100, Wuhan Jiayuan Quantum Dots Co., Ltd, China) and anti-CK mouse monoclonal antibody (Clone: AE1/AE3; dilution: 1:100, Wuhan Jiayuan Quantum Dots Co., Ltd, China) were applied to simultaneously recognize Ki67 and CK antigens in BC tissue sections at 4 °C overnight. This evidence concerns the gene MKI67 and breast cancer.