Although we did not observe a protective effect of PKR deletion on either the development of obesity or glucose intolerance, we nonetheless sought to determine whether the loss of PKR could prevent adipose tissue inflammation, as we recently demonstrated that adipose tissue macrophage recruitment due to HF feeding could be prevented, in the absence of whole-body changes in insulin sensitivity33. This evidence concerns the gene EIF2AK2 and obesity due to melanocortin 4 receptor deficiency.