Multivariate analysis involving sex, age, Astler-Coller stage, tumor grade, loss of MMR proteins and separately E2F1 and TS expression in patients with colon cancer (n = 90) identified only significant correlation of TS+ (HR = 3.96, 95 % CI = 1.19–13.22, P = 0.03), Astler Coller C stage (HR = 3.01, 95 % CI = 1.29–7.02, P = 0.01) and loss of MMR proteins (HR = 0.20, 95 % CI = 0.04–0.86, P = 0.03) with DFS. Here, TYMS is linked to malignant colon neoplasm.