Multivariate analysis involving sex, age, Astler-Coller stage, tumor grade, tumor site, loss of MMR proteins and separately E2F1 and TS expression in all patients (n = 180) identified significant associations only for Astler-Coller stage (C vs. B2) which was significantly associated with worse DFS (HR = 3.01, 95 % CI = 1.66–5.46, P = 0.0003) and OS (HR = 3.24, 95 % CI = 1.49–7.03, P = 0.003), male sex associated with worse OS (HR = 2.05, 95 % CI = 1.04–7.03, P = 0.04) and loss of MMR which was significantly associated with better DFS (HR = 3.71, 95 % CI = 1.74–7.91, P = 0.0007). Here, MRC1 is linked to neoplasm.