To evaluate the C1q-mediated effects, melanoma cells were labelled with the fluorescent probe FAST DiI (Molecular Probes, Invitrogen) and allowed to adhere to plate-bound C1q or a mixture of fibronectin (FN) and C1q, using bovine serum albumin (BSA) and FN as negative and positive controls, respectively. This evidence concerns the gene FN1 and melanoma.