DNAJC5 and malaria: While naturally acquired immune responses have been better characterized in areas of high transmission [35, 36], only a few studies have shown that natural malaria transmission in areas of lower endemicity also induces significant but low ELISpot activities to antigens such as CSP, CelTOS and AMA1 at these sites [25, 27] and that it is possible to measure these reproducibly using positivity criteria of appropriate stringency that was lower than that used to define high vaccine-induced responses.