The importance of LSVs formulation is manifested in how common complex LSVs are indiverse metazoans, making up at least a third of observed LSVs in human and mouse.Complex LSVs are also enriched for regulated splicing when analyzing over thirtydatasets across different tissues, developmental stages, splice factor knockdowns andneurodegenerative disease. This evidence concerns the gene SLU7 and glycogen storage disease VI.