The osteogenic induction with treated dentin matrix medium (TDMM) upregulated the mRNA expression of Alp, Bsp, Opn, Col1, and Tgfb1 (P < 0.01) with various ratios; while these induced upregulations of Alp, Bsp, Opn, and Tgfb1 were inhibited after Clcn7 gene level was lowered by Clcn7 shRNA lentivirus infection (Fig. 4A~C). This evidence concerns the gene SPP1 and lentivirus infection.