RPS6KB1 and nutritional disorder: In an attempt to understand the impact that iron deficiency may have upon mTORC1 signalling, Caco-2 cells maintained in DMEM containing amino acids and FBS were incubated with increasing concentrations of DFO (0–100 μM) for 16 h prior to assessing the phosphorylation status of S6K1, S6 and 4E-BP1.