MYC and cancer: IHC has been shown to provide a good readout of MYC amplification, as a correlation between gene amplification determined by in‐situ hybridization (ISH) and protein expression determined by IHC has been shown in a number of cancers.7, 8, 9, 10, 11 Furthermore, IHC can assist in the identification of cases in which protein overexpression has occurred because of chromosomal rearrangement, upstream mutation, or environmental cues.