PMEL and neoplasm: Thus, with the aim of isolating rare tumour-specific T-cells, a CD8+ T cell library (576 wells at 1000 cells per well) was generated from the PBMC of a healthy HLA-A2+ donor, and screened via IFNγ ELISpot against a pool of five HLA-A2-restricted tumour peptides (melanoma-associated antigen-3 (MAGE-A3)112–120 (Chinnasamy et al., 2011), MAGE-A3240–248 (Graff-Dubois et al., 2002), cadherin-3/P-cadherin (CDH3)655–663 (Imai et al., 2008), NY-BR-1904–912 (Wang et al., 2006) and glycoprotein 100 (gp100)280–288 (Kawakami et al., 1995)).