EN2 and neoplasm: Next, a second CD8+ T-cell library (288 wells at 500 cells per well) was produced from a different healthy HLA-A2+ donor, but this time screened against two separate pools of HLA-A2-restricted tumour peptides, (Pool 1: prostatic acid phosphatase-3 (PAP-3)299–307 (Harada et al., 2003), melanoma-associated antigen-1 (MAGE-A1)278–286 (Pascolo et al., 2001), MAGE-A3112–120, prostein31–39 (Kiessling et al., 2004), insulin-like growth factor 2 mRNA binding protein 3 (IMP-3199–207) (Tomita et al., 2011), and CDH3655–663; Pool 2: six putative peptides from EN2 (EN2-1, -2, -3, -4, -5, and -6)).