PIK3CA and neoplasm: Contrasting with these results, biomarker analysis from BOLERO-2, which involved 3,230 exons of 182 oncogenes and tumor-suppressor genes that were sequenced using next-generation sequencing, on 309 tissue samples, demonstrated a positive treatment effect in favor of everolimus across the various key genetic marker subgroups.[40] In truth, a greater benefit from everolimus treatment was derived in patients with minimal genetic alterations in PIK3CA/PTEN/CCND1 or FGFR1/2 genes combined.