TRIM28 and obesity due to melanocortin 4 receptor deficiency: Mimicking analysis of the Trim28+/D9 haploinsufficient mouse scenario, we sub-divided all individuals into obese or lean groups of comparably high or low TRIM28 levels (Trim28_High and Trim28_Low, respectively) and used qPCR to measure expression of six of our leading-edge, Trim28+/D9-obesity-associated IGN1 genes (Figures 4C and 4D).