Compared to non-operated mice, renal NGAL mRNA (NGAL-mRNA) expression, and plasma and urinary NGAL (pNGAL and uNGAL) protein levels were elevated already by 10-min ischemia and were further elevated significantly from 10-min to more severe 20- or 30-min renal ischemia. This evidence concerns the gene LCN2 and ischemia.