In the other hand, knockdown of OPN in HCC-LM3 and MHCC-97H cell lines (Supplementary Figure 1B and 1C), which are high metastatic [17] and have increased levels of OPN [15, 18], induced an increase in E-cadherin level and significant decreases in the expression levels of N-cadherin, vimentin, and Twist1 (Figure 1B, right). Here, SPP1 is linked to hepatocellular carcinoma.