To further investigate the correlation between miR-129 and Notch-1, the mRNA levels of miR-129 and Notch-1 were detected from 16 clinical high-grade glioma samples (WHO grade III or IV) and two glioma cell lines (U87, U251) compared with 8 normal brain tissues from brain trauma patients. This evidence concerns the gene NOTCH1 and central nervous system cancer.