These data were supported by preliminary spheroid invasion assays (multicellular tumor spheroids (MTS) formed via the spontaneous aggregation of 10,000 cells/well and embedded in 3D rat-tail type-1 collagen matrices), showing that the constitutively active RhoA-expressing MeWo clones exhibited an invasive phenotype, in contrast to the RhoA-deficient MeWo clones (Supplementary Figure S3). This evidence concerns the gene RHOA and neoplasm.