We demonstrated that Twist1 overexpression in the HNSCC cell lines not only mediates the expression of the endothelial-specific markers including CD31 [84], CD144 [85], von Willebrand factor (vWF) [86], Tie2 [87], endoglin (CD105) [88], and intercellular adhesion molecule 1 (ICAM1) [89], but also exhibited obvious ability of capillary-like network formation and the ability of DiI-AcLDL (1,1′-dioctadecyl-3,3,3′,3′-tetramethyl-indocarbocyanide perchlorate-labeled acetylated low density lipoproteins) uptake [90, 91]. This evidence concerns the gene VWF and head and neck squamous cell carcinoma.