Real-time PCR (Fig. 2b) and western blot (Fig. 2c) results showed that both the mRNA and protein levels of both GSK3β and SFRP2 were significantly downregulated in miR-224-overexpressing cells, whereas GSK3β and SFRP2 were upregulated after inhibition of miR-224 in CRC cells. This evidence concerns the gene GSK3B and colorectal carcinoma.