H1299 (non-small cell lung carcinoma) cells stably expressing EGFP-paxillin (marker for CMACs) and RubyRed-LifeAct (marker for F-actin) (H1299 P/L cells [Lock et al., 2014]) were imaged via confocal microscopy on glass coated with 2.5 μg/ml fibronectin (FN). The gene discussed is PXN; the disease is non-small cell lung carcinoma.