NOTCH1 and acute lymphoblastic leukemia: Here, we addressed these issues in T-ALL cells exhibiting mutations in the HD-domain of Notch1 (CCRF-CEM), a doxorubicin-resistant version of these cells (CEM-ADR5000), and Jurkat cells that possess extracellular juxtamembrane expansion mutations of Notch1.18 As a consequence of SERCA pump inhibition, CJ induces calcium release from the endoplasmic reticulum (ER), promoting an increase in reactive oxygen species (ROS) and apoptosis of T-ALL cells.