Similar to those observed in gastric cancer cells [18], annexin V-7AAD binding assay and flow cytometric H2-DCFDA staining further showed that PTGR2-silenced BxPC-3 exerted elevated percentage of both apoptotic and necrotic cells (Fig 3A) as well as induced ROS production (Fig 3B). Here, ANXA5 is linked to gastric cancer.