IDH1 and central nervous system cancer: After gene transfer into F98 glioma cell lines with lentiviral vectors containing IDH1/2–WT–GFP, IDH–R132H/–R172K–GFP, or empty–mock–GFP expression codons (Fig 1A), GFP–expressing F98 cells represented ~95.8–99.1% of the total population (Fig 1B).