To investigate whether reduction of mutant p53 would have an effect on the level of Sp1 or Ets-1 in lung cancer cells we performed Western analysis on H1975 and KNS-62 cells stably expressing shRNA against the endogenous mutant p53 (or GFP as control) and saw that when mutant p53 is knocked-down the level of Sp1, and to some degree Ets-1, is reduced as well (Figure 6F). The gene discussed is TP53; the disease is lung cancer.