Unlike overexpression of lbx genes, lbx1b-/- and lbx2-/- mutant larvae displayed a straight trunk comparable to wild-type larvae (Fig 2D), suggesting the involvement of gain-of-function but not loss-of-function of lbx1b in the body curvature phenotype that might be related to scoliosis susceptibility. This evidence concerns the gene LBX2 and scoliosis.