Remarkably, the IFN-γ released following pulsing these cells with mutant MED15 peptide was comparable to that measured after TILs stimulation by the patient's autologous tumor cells (17,000 pg ml−1 compared to 18,700 pg ml−1 with the autologous melanoma), indicating that the peptide binds the B*51 allele. This evidence concerns the gene IFNG and melanoma.