The TGFBI variant was confirmed by Sanger DNA sequence analysis in the siblings, II.3 and II.5, who had hypocalcemia and keratoconus, but its absence in the sister II.4, who has hypocalcemia only, indicates that this TGFBI variant is not involved in the etiology of hypocalcemia (Supporting Fig. 1A). This evidence concerns the gene TGFBI and Hypocalcemia.