The results demonstrated that HPV-16 E7 DNA-induced E7-specific cellular immune responses and protection of animals from HPV 16 E7-associated tumor growth can be enhanced with α-GalCer and MPL adjuvant combination, as evidenced by significant enhanced lymphocyte proliferation, IFN-γ, Il-12, IL-4, cytotoxicity and in vivo anti-tumor effects, compared with control groups. The gene discussed is IFNG; the disease is neoplasm.