We also found that when the apoptosis-related gene DAPK-1 was methylated in cancerous tissues, the tumor was more likely to be N3 on the TNM classification (tumor metastases found in ≥ 7 regional lymph nodes) than N0–2 (p = 0.05) and to be an advanced tumor (stage III rather than stages I–II; p = 0.05). This evidence concerns the gene DAPK1 and neoplasm.