Consistent results were obtained in cultured cell lines by WB with the results shown in the right panel of Fig. 1b, which reveals that comparing with BEAS-2B cells (a normal alveolar epithelium cell line), DACH1 expression was significantly decreased in both two lung adenocarcinoma cell lines, LTEP-α-2 and A549. This evidence concerns the gene DACH1 and lung adenocarcinoma.