To exclude the possibility of an increase of nonspecific staining after irradiation we chose a tumor that was irradiated with 10 Gy 48 hours prior to PARPi-FL staining, and found a specific localization of the PARPi-FL signal in nuclei (Hoechst staining) which express PARP1 (S2B Fig). This evidence concerns the gene PARP1 and neoplasm.