CLP significantly increased both NF-κB expression and pIκB content in the aorta, compared to the SHAM group, and significantly more in the CLP-AFA than in the CLP-HN and CLP-AF groups (Fig 4A and 4B, S3 Table). Here, NFKB1 is linked to ankyloblepharon filiforme adnatum-cleft palate syndrome.