Experimental metastasis assays showed that Tob1 knockdown suppressed lung colonization of 6DT1 cells (Fig 6K), consistent with Tob1 acting at the same stage of the invasion-metastasis cascade as Cnot7. In 4T1 cells, Tob1 knockdown suppressed tumor mass and pulmonary metastasis (S3 Fig). This evidence concerns the gene TOB1 and neoplasm.