Notably, tumour PT4 demonstrated significant necrosis at the time of necropsy, and sequencing analysis revealed co-enrichment for Insulin-Like Growth Factor Binding Protein 5, IGFBP5R208H, in two cores (C1 and C2) taken from the necrotic region of the tumour compared with a non-necrotic core (C3) fully enriched for NADKI90F (C1, 99%; Supplementary Fig. 3). The gene discussed is IGFBP5; the disease is neoplasm.