NADK and cancer: Depletion of NADK in normal fibroblasts (PC10) using the same three shRNAs (shNADK#4, 8 and 10) cloned into a Dox-inducible shRNA expression vector23 had no effect on proliferation in the presence of Dox compared with off Dox (Fig. 5d and Supplementary Fig. 6a), whereas inducible knockdown of NADK had a marked effect on AsPC-1 cancer cell proliferation by 8 days following Dox addition (Fig. 5d and Supplementary Fig. 6b) consistent with growth in soft agar (Fig. 5c).