To evaluate the co-expression of imprinted genes and miRNAs at the 14q32 locus, we analyzed the expression of DLK1-DIO3 cluster genes (DIO3, DLK1, MEG3, and MEG8) and representative 14q32 miRNAs (miR-134, miR-154, and miR-382) in an independent set of 43 osteosarcoma tumor samples. Here, DLK1 is linked to osteosarcoma.