DLK1 and osteosarcoma: To determine the effects of imprinting defects at 14q32-DMRs on gene expression, we measured the expression levels by qRT-PCR of 6 imprinted genes in this locus including paternal (DLK1, RTL1, and DIO3) and maternal (MEG3, MEG8, and DIOAS) genes expressed in 4 normal bone tissue samples, six 14q-I(−) and eight 14q-I(+) osteosarcoma samples (Figure 3).