We initially examined the effect of PI-103, a dual PI3K/MTOR pathway inhibitor, on cell signalling and autophagy of BRAFV600E bearing colorectal cancer cell lines as shown in Figure 3A (lanes 4, 8, 12), Figure 3B (4th column) and Figure 3E (3rd column). The gene discussed is PIK3CA; the disease is colorectal cancer.