Combination treatments with PLX4720 were also performed for BafA1, in order to examine their potential synergistic effect on BRAFV600E colorectal cancer cell line viability and cell death, as tested by SRB assay and confocal microscopy for Hoechst/Caspase-3, respectively, but no evident synergy was observed as shown in Sup. The gene discussed is CASP3; the disease is colorectal cancer.