Our data emphasise the importance of the PI3-kinase/Akt pathway in oestrogen-responsive breast cancer as the rapid and dramatic loss of FAK phosphorylation within 15 minutes of detachment, followed by PARP cleavage after 1 hour, was associated with a decrease in phosphorylated Akt but an increase in phosphorylated MAP-kinases. Here, PTK2 is linked to breast cancer.