ESR1 and astrocytoma (excluding glioblastoma): ER+ PgR+ Ki-67- breast cancer cells were successfully recognized as nuclei with a purple color (S4E Fig), indicating that light-field chromogenic triple immunostaining using these chromogens is suitable for use in searching for SOX2+ (or NANOG+) HIF-1α+ RNApII-S2P-/low cells in astrocytoma tissues.