The analysis of estrogen receptor profile in xenografts a) confirmed the lack of ERα expression in the epithelial tumor component, thus indicating that tumor formation in vivo was not the results of acquired ERα expression, b) showed no differences between experimental groups in either ERβ1, ERβ5 and GPER protein level, and c) revealed an increased nuclear ERβ2 expression in E2-treated females (in the HEY model only). Here, ESR1 is linked to neoplasm.