Here, we designed the present study aiming to investigate whether NRP-1 expression correlates with the clinicopathologic features of gastric cancer, and whether NRP-1 depletion could inhibit the growth and metastasis of gastric cancer cells, and whether NRP-1 displays its role as co-receptors by interacting the VEGF, EGF and HGF-mediated pathways in gastric cancer cells. Here, AQP1 is linked to gastric cancer.