Pharmaceutical inhibitors against Rho-associated, coiled-coil containing protein kinase (ROCK) and myosin light chain kinase (MLCK) shows that migration of metastatic breast cancer cells, MDA-MB-231, along 3D collagen fiber is dependent on Rho- and ROCK-associated actomyosin contractility but not on MLCK signaling15. Here, MYLK is linked to breast cancer.