Since standard two-dimensional gel electrophoresis and in-gel digestion had failed to identify the dystrophin-glycoprotein complex during early proteomic studies of muscular dystrophy [23], [25], a combination of one-dimensional gradient gel electrophoresis, electrophoretic protein transfer and on-membrane digestion were applied prior to mass spectrometric analysis [86]. The gene discussed is DMD; the disease is muscular dystrophy.