Of 100 protein and phospho-specific antibodies used in RPPA of lysates from tumor cell lines (PC3, prostate; U87, glioblastoma; MCF7.1, breast), p-Akt(Ser473) and p-Akt(Thr308) were elevated after treatment as expected, whereas substrates downstream of Akt, PRAS40, GSK-3β, mTOR, eIF4G, ATP citrate lyase, FoxO3A and S6, showed decreased phosphorylation. This evidence concerns the gene ACLY and glioblastoma.