AKT1 and anaplastic cancer: However, when Akt1 immunoprecipitates were examined by immunoblotting, the ratio of p-Akt(Ser473) to total Akt1 was lower in cancers compared to normal tissues (although only a subset of the tumors were shown to be analyzed in this way and it is unclear if the anaplastic cancers with high nuclear Akt1 were included).