For the HP1α-V3 to STET mRNA expression ratio, significant differences were observed for both primary carcinoma samples both from patients with metastasis (1.81-fold, P < 0.01) and without metastasis (1.68-fold, P < 0.01) compared to lymph node metastases (Additional file 6: Figure S4). The gene discussed is CBX5; the disease is carcinoma.