CRH and Alzheimer disease: Western blot analysis was used to examine levels of tau-P at the AD-relevant C-terminal site (PHF-1) in extracts from hippocampal neuronal cultures after 0, 0.5, 2, 4, 8 or 24hr of 1 μm (low) or 10 μm (high) CRF treatment (Fig 1A and 1B).