This study demonstrates that a diet with high amount of simple carbohydrates, which resembles the current human dietary pattern, was able to induce obesity-related NAFLD, here characterized histologically by hepatic steatosis and hepatocyte ballooning, clinically by increased TC and BMI associated with hyperleptinemia, and metabolically by hyperglycemia, hyperinsulinemia (with subsequent insulin return to baseline levels), hypertriglyceridemia, increased serum levels of VLDL-cholesterol, depletion of antioxidants liver enzymes, and increased levels of MDA, an oxidative stress marker. Here, INS is linked to obesity due to melanocortin 4 receptor deficiency.