FLI1 and melanoma: In this study, we used transparent zebrafish Tg(fli1:EGFP) or Tg(kdr1:mCherry) to image metastatic processes with high-resolution microscopy after mouse melanoma B16 cells expressing KatushkaS158A, a tetrameric far-red fluorescent protein (tfRFP)17, or B16 cells transfected with YFP (yellow fluorescent protein) or CFP (cyan fluorescent protein), are injected into the yolk (or pericardium cavity) of the developing zebrafish 2 days post fertilization (2 dpf).