SOX2 and melanoma: Comparing these data in Fig. 5 with those in Fig. 3 reveal that Sox2 shRNA treated TRCs behaved quantitatively similar to those untreated melanoma cells grown on 2D rigid plastic (Supplementary Fig. 7), further strengthening the finding that Sox2 is critical in the dynamic processes of metastasis in vivo by melanoma cells, in addition to its essential roles in self-renewal12, 13.