First of all, to explore the anti-tumor effect of AZD3463 on NB cell lines, we exposed four ALK wild type cell lines (SK-N-AS, IMR-32, NGP, NB-19) and two ALK mutant cell lines (LA-N-6 (D1091N) and SH-SY5Y (WT/F1174L))10, 29 to increased concentrations of AZD3463. This evidence concerns the gene ALK and neoplasm.