Meanwhile, 808 nm laser irradiation alone did not induce obvious cytotoxicity in both HT-29 and NIH/3T3 cells (Supplementary Fig. 17), indicating that the covalent cross-linking of CRUN triggered by specific enzyme would increase the singlet oxygen generation, and thus induce the enhanced cell death in the targeted tumour cells with overexpression of the CstB enzyme. Here, CSTB is linked to neoplasm.